Huma DermiMatrix Coat, Native Human-Derived Skin ECM, 1 mg/ml
Huma DermiMatrix Coat
Native Human-Derived Skin ECM, 1 mg/ml
SKU: HSMS
Huma DermiMatrix Coat is extracted from nutrient-rich human skin tissue sourced strictly from tissue partners who comply with the requirements of transplantable human tissue. Huma DermiMatrix Coat supports various cell types, including fibroblasts.
Applications:
Background
Product Description
Huma DermiMatrix Coat is a native human-derived skin extracellular matrix (ECM) product. Due to its human origin and minimal processing, Huma DermiMatrix Coat retains the native matrix proteins and growth factors found in human skin including collagens (Type I, III, IV), elastin, glycoproteins (laminin, fibronectin, tenascin, fibrillin), and glycosaminoglycans (hyaluronic acid, chondroitin sulfate, dermatan sulfate, heparan sulfate, and heparin).
Huma DermiMatrix Coat is ideal for many applications including coating tissue culture surfaces to support cell attachment. Huma DermiMatrix Coat supports various cell types, including keratinocytes, fibroblasts, melanocytes, adipocytes, endothelial cells, and more.
Tissue Source
Huma DermiMatrix Coat is obtained from human tissue that has been tested and found negative for a minimum of HIV-1, HIV-2, hepatitis B, and hepatitis C, as well as other infectious agents. Please review the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. Huma DermiMatrix Coat is for research use only and is not intended for human use, diagnosis, screening, household, food or other uses
Precautions and Disclaimer
Huma DermiMatrix Coat is obtained from human tissue that has been tested and found negative for minimum of HIV-1 and -2, hepatitis B, and hepatitis C, as well as other infectious agents. Please review the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. Huma DermiMatrix Coat is for research use only and is not intended for human use, diagnosis, screening, household, food or other uses.
Storage Conditions/Expiration
Huma DermiMatrix Coat should be stored at -80 °C upon receipt. Huma DermiMatrix Coat is composed of stable proteins if it is stored frozen until use while minimizing freeze-thaw cycles. Research grade products have a shelf life of 6 months upon receipt.
Instructions for Use
Huma DermiMatrix Coat-Coated Tissue Cultureware
Note: The following are general recommendations. Researcher should optimize parameters based on their specific applications and cell types.
Optimal ECM concentrations for tissue culture surface coatings may depend on application and cell type and must be determined for each application. Typically, 10 – 100 µg/ml of ECM is used.
- Thaw Huma DermiMatrix Coat in the fridge overnight. Aliquot Huma DermiMatrix Coat and freeze at -80°C to minimize freeze-thaw cycles.
- Aliquot DermiMatrix Coat and freeze to minimize freeze-thaw cycles.
- Dilute Huma DermiMatrix Coat in 10-20 mM HCl with a pH of 1.9-2.1 to a concentration of 0.1 mg/ml. It is recommended to use ultrapure sterile water to make the 10-20 mM HCl.
- Aseptically add ECM solution to tissue culture surface to evenly cover entire surface.
- Cover and incubate ECM coated surfaces at 37 °C for at least 2 hours. The optimal incubation time may vary depending on the experiment and cell type and should be determined for each application.
- Transfer ECM coated surfaces to biosafety cabinet and aspirate excess solution from coated surface careful not to scratch the surface. Remove lid to allow for air drying overnight.
- Rinse the coated culture surface with sterile PBS or culture medium to remove any residual acid.
- Use coated cultureware immediately or keep sterile and store at 2-10 °C.
Quality Testing
| Test | Specification |
|---|---|
| Appearance (form) | |
| Form | Solution |
| Solution Turbidity | Clear to Opaque |
| pH | 1.9-2.1 |
| Cell Attachment | Pass |
| Selected Composition of Main Matrix Proteins by Qualitative Analysis*
Collagens (Type I, III, IV), elastin, glycoproteins (laminin, fibronectin, tenascin, fibrillin), and glycosaminoglycans (hyaluronic acid, chondroitin sulfate, dermatan sulfate, heparan sulfate, and heparin) |
Pass |
| Immunology/Serology Testing** | |
| HBV | Non-Reactive |
| HCV | Non-Reactive |
| HIV-1 | Non-Reactive |
| HIV-2 | Non-Reactive |
| RPR-Syphilis | Non-Reactive |
* Gel data is available upon request
** Testing was done using FDA approved kits in CLIA certified lab
*** Donor info is available upon request
Safety Data Sheet