HumaElastin – Human Skin Elastin, Lyophilized
HumaElastin
Human Skin Elastin, Lyophilized, 2 mg
SKU: HEL
HumaElastin is extracted from nutrient-rich human skin tissue sourced strictly from tissue partners who comply with the requirements of transplantable human tissue. HumaElastin is ideal for many applications including coating tissue culture surfaces to support cell attachment.
Applications:
Background
Product Description
Elastin is a crucial structural protein found in various connective tissues throughout the body, providing elasticity and resilience to organs and tissues that require the ability to stretch and recoil [1]. It is primarily present in tissues such as the skin, blood vessels, lungs, and ligaments. Elastin is composed of amino acids – predominantly glycine, valine, alanine, and proline, with unique cross-linking patterns that contribute to its distinctive properties [2].
HumaElastin is ideal for many applications including coating tissue culture surfaces to support cell attachment.
Tissue Source
HumaElastin is isolated from human tissue sourced strictly from American Association of Tissue Banks (AATB) accredited and FDA registered tissue banks and organ procurement organizations (OPOs). Humabiologics strives to meet research needs by providing high quality biomaterials obtained from tissue partners who comply with requirements for transplantable human tissues under 21 CFR 1271 of the FDA.
Precautions and Disclaimer
HumaElastin is obtained from human tissue that has been tested and found negative for a minimum of HIV-1, HIV-2, hepatitis B, and hepatitis C, as well as other infectious agents. Please review the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. HumaElastin is for research use only and is not intended for human use, diagnosis, screening, household, food, or other uses.
Storage Conditions/Expiration
HumaElastin should be stored at -20 °C upon receipt. HumaElastin is composed of stable proteins if it is stored frozen until use while minimizing freeze-thaw cycles. Research grade products have a shelf-life of 6 months upon receipt.
References
- Kielty, C. M., & Sherratt, M. J. (2004). Elastic fibres. Journal of cell science, 117(4), 4855-4857.
- Mecham, R. P. (2008). Overview of Extracellular Matrix. Current protocols in cell biology, 4.10.1-4.10.16.
Instructions for Use
HumaElastin-Coated Tissue Cultureware
Note: The following are general recommendations. Researchers should optimize parameters based on their specific applications and cell types.
Optimal elastin concentrations for tissue culture surface coatings may depend on application and cell type and must be determined for each application. Typically, 10 – 100 µg/ml of elastin is used.
- Thaw HumaElastin in the fridge overnight. Aliquot HumaElastin and freeze to minimize freeze-thaw cycles.
- Dilute HumaElastin in 10 – 20 mM HCl with a pH of 1.9-2.1 to a concentration of 0.1 mg/ml. It is recommended to use ultrapure sterile water to make the HCl.
- Aseptically add elastin solution to tissue culture surface to evenly cover entire surface.
- Cover and incubate elastin coated surfaces at 37 °C for at least 2 hours. The optimal incubation time may vary depending on the experiment and cell type and should be determined for each application.
- Transfer elastin coated surfaces to biosafety cabinet and aspirate excess solution from coated surface careful not to scratch the surface. Remove lid to allow for air drying overnight.
- Rinse the coated culture surface with sterile PBS or culture medium to remove residual acid.
- Use coated cultureware immediately or keep sterile and store at 2-10 °C.
Quality Testing
| Test | Specification |
|---|---|
| Appearance (form) | |
| Color | White |
| Form | Fibrous Flaky Material |
| Solution Turbidity | Clear to Slightly Opaque |
| pH (1 mg/ml solution in 15 mM HCl) | 1.9-2.1 |
| Cell Attachment | Pass |
| Immunology/Serology Testing** | |
| HBV | Non-Reactive |
| HCV | Non-Reactive |
| HIV-1 | Non-Reactive |
| HIV-2 | Non-Reactive |
| RPR-Syphilis | Non-Reactive |
** Testing was done using FDA approved kits in CLIA certified lab
*** Donor info is available upon request
Publications
Safety Data Sheet