HumaDerm® IV- Human Collagen Type IV, Lyophilized

HumaDerm® IV

Human Collagen Type IV, Lyophilized


HumaDerm® IV is extracted from nutrient-rich human birth tissue sourced strictly from tissue partners who comply with the requirements of transplantable human tissue. HumaDerm® IV is ideal for many applications including coating tissue culture surfaces or microbeads with a thin layer of collagen to support rapid cell attachment and growth. The optimal collagen concentration used may vary depending on cell type and application.


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Product Description

Type IV collagen occupies a distinct role within the realm of connective tissues. It is primarily associated with basement membranes, where it plays a pivotal role in mechanical stability and regulates critical functions including cell growth, differentiation, morphogenesis, homeostasis, and regeneration [1]. This collagen type is vital for various physiological processes, including filtration in the kidneys, scaffolding in tissues, and the formation of blood vessel walls.
Type IV collagen can exist in at least three hetero-trimeric triple helical forms made up of three α chains, each about 180kDa in molecular weight [2,3]. HumaDerm® IV demonstrates the presence of intact α chains on SDS-PAGE. HumaDerm® IV is ideal for many applications including coating tissue culture surfaces to support cell attachment.

Tissue Source

HumaDerm® IV is isolated from human tissue sourced strictly from American Association of Tissue Banks (AATB) accredited and FDA registered tissue banks and organ procurement organizations (OPOs). Humabiologics strives to meet research needs by providing high quality biomaterials obtained from tissue partners who comply with requirements for transplantable human tissues under 21 CFR 1271 of the FDA.

Precautions and Disclaimer

HumaDerm® IV is obtained from human tissue that has been tested and found negative for a minimum of HIV-1, HIV-2, hepatitis B, and hepatitis C, as well as other infectious agents. Please review the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. HumaDerm® IV is for research use only and is not intended for human use, diagnosis, screening, household, food, or other uses.

Storage Conditions

HumaDerm® IV should be stored at -20 °C upon receipt. HumaDerm® IV is composed of stable proteins if it is stored frozen until use while minimizing freeze-thaw cycles. Research grade products have a shelf life of 6 months upon receipt.


  1. Johansson, C., Butkow, R., Wieslander, J. (1992). The Structural Organization of Type IV Collagen. The Journal of Biological Chemistry. Vol. 261, No. 34, pp. 24533-24537.
  2. Triieb, B., Grobli, B., Spies, M., Odermatt, B.F., Winterhalter, K., (1981) Basement Membrane (Type IV) Collagen Is a Heteropolymer. The Journal of Biological Chemistry. Vol. 257, No. 9. pp. 52394245
  3. Bächinger, HP., Mizuno, K., Vranka, JA., Boudko, SP. (2010). Collagen Formation and Structure. Comprehensive Natural Products II, Elsevier, Pages 469-530, ISBN 9780080453828,

HumaDerm IV – Coated Tissue Cultureware

Note: The following are general recommendations. Researcher should optimize parameters based on their specific applications

Optimal collagen concentrations for tissue culture surface coatings may depend on application and cell type and must be determined for each application. Typically, 10 – 100 μg/ml of collagen is used.

  1.  Reconstitute HumaDermTM IV in 10-20 mM HCl with a pH of 1.9-2.1 to the desired concentration. For cell culture coating applications, a concentration of 0.1 mg/ml is recommended. It is recommended to use ultrapure sterile water to make the 10-20 mM HCl.
  2. Mix by shaking or vortexing until the solution is homogeneous.
  3. Aseptically add collagen solution to tissue culture surface to evenly cover entire surface.
  4. Cover and incubate collagen coated surfaces at 37 °C for at least 2 hours. The optimal incubation time may vary depending on the experiment and cell type and should be determined for each application.
  5. Transfer collagen coated surfaces to biosafety cabinet and aspirate excess solution from coated surface careful not to scratch the surface. Remove lid to allow for air drying overnight.
  6. Rinse the coated culture surface with sterile PBS or culture medium to remove residual acid.
  7. Use coated cultureware immediately or keep sterile and store at 2-10 °C.
Test Specification
Appearance (form)
          Color Clear
          Form Solution
          Solution Turbidity Clear to Slightly Opaque
pH 1.9-2.1
Cell Attachment Pass
          SDS-PAGE & Coomassie Stain*
Immunology/Serology Testing**
          HBV Non-Reactive
          HCV Non-Reactive
          HIV-1 Non-Reactive
          HIV-2 Non-Reactive
          RPR-Syphilis Non-Reactive

* Gel data is available upon request 

** Testing was done using FDA approved kits in CLIA certified lab 

*** Donor info is available upon request