HumaDerm® II- Human Collagen Type II, Solution, 1 mg/ml
HumaDerm® II
Human Collagen Type II, Solution, 1 mg/ml
SKU: CCIS
HumaDerm® II is extracted from nutrient-rich human cartilage tissue sourced strictly from tissue partners who comply with the requirements of transplantable human tissue. HumaDerm® II is ideal for many applications including coating tissue culture surfaces to support rapid cell attachment and growth. The optimal concentration may vary depending on cell type.
Applications:
Background
Product Description
Type II collagen is predominantly found in cartilage, the connective tissue that provides structural support and cushioning to joints. It is a critical component of the extracellular matrix in cartilage and plays a significant role in maintaining its structural integrity and function [2]. Type II collagen is a homotrimer composed of three identical α1(II) chains, forming a triple helix structure [1]. It is characterized by the presence of a full-length α chain on SDS-PAGE. Each α1(II) chain is approximately 140 kDa in molecular weight [3]. HumaDerm® II exhibits the presence of the full-length α chain with minimal fragmented proteins present. HumaDerm® II is ideal for many applications including coating tissue culture surfaces to support cell attachment.
Tissue Source
HumaDerm® II is isolated from human tissue sourced strictly from American Association of Tissue Banks (AATB) accredited and FDA registered tissue banks and organ procurement organizations (OPOs). Humabiologics strives to meet research needs by providing high quality biomaterials obtained from tissue partners who comply with requirements for transplantable human tissues under 21 CFR 1271 of the FDA.
Precautions and Disclaimers
HumaDerm® II is obtained from human tissue that has been tested and found negative for a minimum of HIV-1, HIV-2, hepatitis B, and hepatitis C, as well as other infectious agents. Please review the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. HumaDerm® II is for research use only and is not intended for human use, diagnosis, screening, household, food, or other uses.
Storage
HumaDerm® II should be stored at -20 °C upon receipt. HumaDerm® II is composed of stable proteins if it is stored frozen until use while minimizing freeze-thaw cycles. Research grade products have a shelf life of 6 months upon receipt.
References
- Alcaide-Ruggiero, L., Molina-Hernández, V., Granados, M. M., & Domínguez, J. M. (2021). Main and Minor Types of Collagens in the Articular Cartilage: The Role of Collagens in Repair Tissue Evaluation in Chondral Defects. International journal of molecular sciences, 22(24), 13329. https://doi.org/10.3390/ijms222413329
- Bielajew, B.J.; Hu, J.C.; Athanasiou, K.A. (2020). Collagen: Quantification, biomechanics, and role of minor subtypes in cartilage. Nat. Rev. Mater. 2020, 5, 730–747.
- Gelse, K., Pöschl, E., & Aigner, T. (2003). Collagens—structure, function, and biosynthesis. Advanced Drug Delivery Reviews, 55(12), 1531-1546. doi: 10.1016/j.addr.2003.08.002.
Instructions for Use
HumaDerm II – Coated Cultureware
Note: The following are general recommendations. Researcher should optimize parameters based on their specific applications.
Optimal collagen concentrations for tissue culture surface coatings may depend on application and cell type and must be determined for each application. Typically, 10 – 100 µg/ml of collagen is used.
- Thaw HumaDerm II in the fridge overnight.
- Centrifuge HumaDerm II for 1 minute.
- Aliquot HumaDerm II and freeze at -20°C to minimize freeze-thaw cycles.
- Dilute HumaDerm II with 10-20 mM HCl with a pH of 1.9-2.1 to 0.1 mg/ml. It is recommended to use ultrapure sterile water to make the 10-20 mM HCl.
- Mix by shaking or vortexing until the solution is homogeneous.
- Aseptically add collagen solution to tissue culture surface to evenly cover entire surface.
- Cover and incubate collagen coated surfaces at 37 °C for at least 2 hours. The optimal incubation time may vary depending on the experiment and cell type and should be determined for each application.
- Transfer collagen coated surfaces to biosafety cabinet and aspirate excess solution from coated surface careful not to scratch the surface. Remove lid to allow for air drying overnight.
- Rinse the coated culture surface with sterile PBS or culture medium to remove any residual acid.
- Use coated cultureware immediately or keep sterile and store at 2-10 °C.
Quality Testing
| Test | Specification |
|---|---|
| Appearance (form) | |
| Form | Solution |
| Solution Turbidity | Clear to Slightly Opaque |
| pH | 1.9-2.1 |
| Cell Attachment | Pass |
| Purity SDS-PAGE & Coomassie Stain* |
Pass |
| Immunology/Serology Testing** | |
| HBV | Non-Reactive |
| HCV | Non-Reactive |
| HIV-1 | Non-Reactive |
| HIV-2 | Non-Reactive |
| RPR-Syphilis | Non-Reactive |
* Gel data is available upon request
** Testing was done using FDA approved kits in CLIA certified lab
*** Donor info is available upon request
Publications
Safety Data Sheet