HumaDerm® I/III- Human Collagen Type I/III, Lyophilized
HumaDerm® I/III
Human Collagen Type I/III, Lyophilized
SKU: PVIIIIL-10mg
HumaDerm® I/III is extracted from nutrient-rich human birth tissue sourced strictly from tissue partners who comply with the requirements of transplantable human tissue. HumaDerm® I/III is ideal for many applications including coating tissue culture surfaces to support rapid cell attachment and growth. The optimal concentration may vary depending on cell type.
Applications:
Background
Product Description
Type III collagen is known to be associated with Type I Collagen. Collagen type I builds a scaffold with thick fibers that have a low turnover rate. The maturation of collagen type I, however, depends on collagen type III, which produces thin, less durable fibers with a high turnover [1]. Type III collagen conjugated with Type I collagen presents both one α2(I) chain, two α1(I) chains, and three α1(III) chains [11]. HumaDerm® I/III is ideal for many applications including coating tissue culture surfaces to support cell attachment.
Tissue Source
HumaDerm® I/III is isolated from human tissue sourced strictly from American Association of Tissue Banks (AATB) accredited and FDA registered tissue banks and organ procurement organizations (OPOs). Humabiologics strives to meet research needs by providing high quality biomaterials obtained from tissue partners who comply with requirements for transplantable human tissues under 21 CFR 1271 of the FDA.
Precautions and Disclaimers
HumaDerm® I/III is obtained from human tissue that has been tested and found negative for a minimum of HIV-1, HIV-2, hepatitis B, and hepatitis C, as well as other infectious agents. Please review the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. HumaDerm® I/III is for research use only and is not intended for human use, diagnosis, screening, household, food, or other uses.
Storage
HumaDerm® I/III should be stored at -20 °C upon receipt. HumaDerm® I/III is composed of stable proteins if it is stored frozen until use while minimizing freeze-thaw cycles. Research grade products have a shelf life of 6 months upon receipt.
References
- Singh D, Rai V, Agrawal DK. Regulation of Collagen I and Collagen III in Tissue Injury and Regeneration. Cardiol Cardiovasc Med. 2023;7(1):5-16.
doi: 10.26502/fccm.92920302. Epub 2023 Jan 20. PMID: 36776717; PMCID: PMC9912297.
Instructions for Use
HumaDerm I/III – Coated Cultureware
Note: The following are general recommendations. Researcher should optimize parameters based on their specific applications.
Optimal collagen concentrations for tissue culture surface coatings may depend on application and cell type and must be determined for each application. Typically, 10 – 100 µg/ml of collagen is used.
- Thaw HumaDerm I/III in the fridge overnight.
- Centrifuge HumaDerm I/III for 1 minute.
- Aliquot HumaDerm I/III and freeze at -20°C to minimize freeze-thaw cycles.
- Dilute HumaDerm I/III with 10-20 mM HCl with a pH of 1.9-2.1 to 0.1 mg/ml. It is recommended to use ultrapure sterile water to make the 10-20 mM HCl.
- Mix by shaking or vortexing until the solution is homogeneous.
- Aseptically add collagen solution to tissue culture surface to evenly cover entire surface.
- Cover and incubate collagen coated surfaces at 37 °C for at least 2 hours. The optimal incubation time may vary depending on the experiment and cell type and should be determined for each application.
- Transfer collagen coated surfaces to biosafety cabinet and aspirate excess solution from coated surface careful not to scratch the surface. Remove lid to allow for air drying overnight.
- Rinse the coated culture surface with sterile PBS or culture medium to remove any residual acid.
- Use coated cultureware immediately or keep sterile and store at 2-10 °C.
Quality Testing
| Test | Specification |
|---|---|
| Appearance (form) | |
| Form | Solution |
| Solution Turbidity | Clear to Slightly Opaque |
| pH | 1.9-2.1 |
| Cell Attachment | Pass |
| Purity SDS-PAGE & Coomassie Stain* |
Pass |
| Immunology/Serology Testing** | |
| HBV | Non-Reactive |
| HCV | Non-Reactive |
| HIV-1 | Non-Reactive |
| HIV-2 | Non-Reactive |
| RPR-Syphilis | Non-Reactive |
* Gel data is available upon request
** Testing was done using FDA approved kits in CLIA certified lab
*** Donor info is available upon request
Publications
Safety Data Sheet