Huma CardioMatrix Coat, Native Human-Derived Heart ECM, 1 mg/ml
Huma CardioMatrix Coat
Native Human-Derived Heart ECM, 1 mg/ml
SKU: HHMS
Huma CardioMatrix Coat is extracted from human heart tissue sourced strictly from tissue partners who comply with the requirements of transplantable human tissue. Huma CardioMatrix Coat supports various cell types, including cardiomyocytes.
Applications:
Background
Product Description
Huma CardioMatrix Coat is a native human-derived heart extracellular matrix (ECM) product. Due to its human origin and minimal processing, Huma CardioMatrix Coat retains the native matrix proteins and growth factors found in human heart tissue.
Huma CardioMatrix Coat is ideal for many applications including coating tissue culture surfaces to support cell attachment. Huma CardioMatrix Coat supports various cell types, including myocytes, cardiomyocytes, and more.
Tissue Source
Huma CardioMatrix Coat is isolated from human tissue sourced strictly from American Association of Tissue Banks (AATB) accredited and FDA registered tissue banks and organ procurement organizations (OPOs). Humabiologics strives to meet research needs by providing high quality biomaterials obtained from tissue partners who comply with requirements for transplantable human tissues under 21 CFR 1271 of the FDA.
Precautions and Disclaimer
Huma CardioMatrix Coat is obtained from human tissue that has been tested and found negative for a minimum of HIV-1, HIV-2, hepatitis B, and hepatitis C, as well as other infectious agents. Please review the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. Huma CardioMatrix Coat is for research use only and is not intended for human use, diagnosis, screening, household, food, or other uses.
Storage Conditions
Huma CardioMatrix Coat should be stored at -80 °C upon receipt. Huma CardioMatrix Coat is composed of stable proteins if it is stored frozen until use while minimizing freeze-thaw cycles. Research grade products have a shelf life of 6 months upon receipt.
Instructions for Use
Huma CardioMatrix Coat-Coated Tissue Cultureware
Note: The following are general recommendations. Researcher should optimize parameters based on their specific applications and cell types.
Optimal ECM concentrations for tissue culture surface coatings may depend on application and cell type and must be determined for each application. Typically, 10 – 100 µg/ml of ECM is used.
- Thaw Huma CardioMatrix Coat in the fridge overnight.
- Aliquot Huma CardioMatrix Coat and freeze to minimize freeze-thaw cycles.
- Dilute Huma CardioMatrix Coat in 10 – 20 mM HCl with a pH of 1.9-2.1 to a concentration of 0.1 mg/ml. It is recommended to use ultrapure sterile water to make the HCl.
- Aseptically add ECM solution to tissue culture surface to evenly cover entire surface.
- Cover and incubate ECM coated surfaces at 37 °C for at least 2 hours. The optimal incubation time may vary depending on the experiment and cell type and should be determined for each application.
- Transfer ECM coated surfaces to biosafety cabinet and aspirate excess solution from coated surface careful not to scratch the surface. Remove lid to allow for air drying overnight.
- Rinse the coated culture surface with sterile PBS or culture medium to remove residual acid.
- Use coated cultureware immediately or keep sterile and store at 2-10 °C
Quality Testing
| Test | Specification |
|---|---|
| Appearance (form) | |
| Form | Solution |
| Solution Turbidity | Clear to Opaque |
| pH | 1.9-2.1 |
| Cell Attachment | Pass |
| Selected Composition of Main Matrix Proteins by Qualitative Analysis*
Collagens (Type I, III, IV, VI), glycoproteins (laminin, fibronectin, entactin, fibrillin, tenascin), and glycosaminoglycans (heparan sulfate, hyaluronic acid, chondroitin sulfate and dermatan sulfate) |
Pass |
| Immunology/Serology Testing** | |
| HBV | Non-Reactive |
| HCV | Non-Reactive |
| HIV-1 | Non-Reactive |
| HIV-2 | Non-Reactive |
| RPR-Syphilis | Non-Reactive |
* Gel data is available upon request
** Testing was done using FDA approved kits in CLIA certified lab
*** Donor info is available upon request
Publications
Safety Data Sheet